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KMID : 1098420140220060449
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Korean Journal of Medicinal Crop Science
2014 Volume.22 No. 6 p.449 ~ p.456
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Anti-inflammatory Activity of Solvent Fractions from Ginseng Berry Extract in LPS-Induced RAW264.7 Cells
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Lee Ka-Soon
Kim Gwan-Hou
Seong Bong-Jae
Kim Sun-Ick
Han Seung-Ho
Lee Sox-Su
Yang Hui
Yoo Yung-Choon
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Abstract
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Anti-inflammatory activity of the extracts of ginseng berry (GBE) was investigated through the evaluation of its inhibitory effect on the production of inflammatory meditator, nitric oxide(NO), tumor necrocis factor-alpha (TNF-α), interleukin-6 (IL-6) in LPS-induced RAW264.7 macrophage cells. GBE was fractionated using n-hexane, chloroform, ethylacetate, buthanol and H2O, sequentially. RAW264.7 cells were induced 100 ng/㎖ of lipopolysaccharide (LPS) and treated with 0, 1.6, 8, 40 and 200 ㎍/㎖ of GBE fractions. LPS-induced NO production on all of GBE fractions was inhibited with increasing added concentration of GBE fractions. Chloroform fraction of GBE was the most effective in inhibiting LPSinduced TNF-α production. Hexane, chloroform and H2O fractions of GBE exhibit strong inhibition LPS-induced IL-6 production. Especially, H2O fractions of GBE was the most effective in inhibiting LPD-induced IL-6 production without significant cytotoxicity in RAW264.7 cells, and reduced the activation of mitogen-activated protein kinases (MAPK) and IkB
phosphorylation. These results indicate that H2O fractions of GBE exhibits strong anti-inflammatory effects by inhibition of
NF-kB by inhibition of p-38 on MAPK and IkB phosphorylation.
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KEYWORD
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Ginseng Berry, Solvent Fractions, Anti-Inflammation, Nitric Oxide, Cytokines
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